(NewsNation) — There will be no “virgin birth” or “sharkrays.” Charlotte, the stingray that showed signs of pregnancy earlier this year, is not pregnant, according to the owners of the aquarium where she resides.

Brenda Ramer, the owner of Aquarium & Shark Lab by Team ECCO in Hendersonville, N.C., told an Asheville TV station that Charlotte has a reproductive disease that “negatively impacted her reproductive system.”

A Facebook post by the aquarium did not mention Charlotte’s purported pregnancy but called the findings “truly a sad and unexpected medical development.”

Reproductive disease is common in older female rays in captivity and is already a topic of interest for aquatic vets and scientists.

In February, speculation was rampant about the female stingray that hasn’t been with a male ray in many years.

The aquarium said Charlotte was the first round stingray to display parthenogenesis, or reproduction without fertilization of an egg.

According to The National Geographic, parthenogenesis is common among many small invertebrates and fish, and has been documented in more than 80 species

Some wild theorists wondered if the “father” was one of two male sharks that share her tank. But that, say scientists, is physically impossible due to the species’ incompatible DNA.

As for Charlotte’s prognosis, the aquarium said its “priority is to focus on Charlotte’s health and well-being. We will work with, and be guided by, veterinarians and specialists to better understand this disease and the treatment options for Charlotte.”