TORONTO (NewsNation Now) — A blockade of the bridge between Canada and Detroit by protesters demanding an end to Canada’s COVID-19 restrictions forced the shutdown of a Ford plant and began to have broader implications for the North American auto industry.

Prime Minister Justin Trudeau, meanwhile, stood firm against an easing of Canada’s COVID-19 restrictions in the face of mounting pressure during recent weeks by protests against the restrictions and against Trudeau himself.

The protest by people mostly in pickup trucks entered its third day at the Ambassador Bridge between Detroit and Windsor, Ontario. Traffic was prevented from entering Canada, while U.S.-bound traffic was still moving.

“There’s nothing organized about it. It’s just a grassroots thing where people are helping people and trying to get change,” Canadian trucker Jason Vanderwier told NewsNation.

The bridge carries 25% of all trade between the two countries, and Canadian authorities expressed increasing worry about the economic effects.

On Thursday, General Motors confirmed to NewsNation it has canceled two shifts at one Michigan factory and has been rerouting trucks to keep another plant running amid a parts shortages.

Late Wednesday Ford said that parts shortages forced it to shut down its engine plant in Windsor and to run an assembly plant in Oakville, Ontario, on a reduced schedule.

“This interruption on the Detroit-Windsor bridge hurts customers, auto workers, suppliers, communities and companies on both sides of the border,” Ford said in a statement. “We hope this situation is resolved quickly because it could have widespread impact on all automakers in the U.S. and Canada.”

Brian Kingston, CEO of Canadian Vehicle Manufacturers Association says the longer the protests persists it will create “problems throughout the supply chain, delays in getting parts and components to the assembly plants, and will ultimately have an impact on jobs.”

Shortages due to the blockade also forced General Motors to cancel the second shift of the day at its midsize-SUV factory near Lansing, Michigan. Spokesman Dan Flores said it was expected to restart Thursday and no additional impact was expected for the time being.

Later Wednesday, Toyota spokesman Scott Vazin said the company will not be able to manufacture anything at three Canadian plants for the rest of this week due to parts shortages. A statement attributed the problem to supply chain, weather and pandemic-related challenges, but the shutdowns came just days after the blockade began Monday.

“Our teams are working diligently to minimize the impact on production,” the company said, adding that it doesn’t expect any layoffs at this time.

Stellantis, formerly Fiat Chrysler, reported normal operations, though the company had to cut shifts short the previous day at its Windsor minivan plant.

A growing number of Canadian provinces have moved to lift some of their precautions as the omicron surge levels off, but Trudeau defended the measures the federal government is responsible for, including the one that has angered many truck drivers: a rule that took effect Jan. 15 requiring truckers entering Canada to be fully vaccinated.

About 90% of truckers in Canada are vaccinated, and trucker associations and many big-rig operators have denounced the protests. The U.S. has the same vaccination rule for truckers entering the country, so it would make little difference if Trudeau lifted the restriction.

Protesters have also been blocking the border crossing at Coutts, Alberta, for a week and a half, with about 50 trucks remaining there Wednesday. And more than 400 trucks have paralyzed downtown Ottawa, Canada’s capital, in a protest that began late last month.

“We don’t want to see the military coming in but at the same time if they refuse to leave I’m not sure what the options are,” said Canadian resident Theresa Forman.

While protesters have been calling for Trudeau’s removal, most of the restrictive measures around the country have been put in place by provincial governments. Those include requirements that people show proof-of-vaccination “passports” to enter restaurants, gyms, movie theaters and sporting events.

Alberta, Saskatchewan, Quebec, Prince Edward Island and Nova Scotia announced plans this week to roll back some or all of their precautions. Alberta, Canada’s most conservative province, dropped its vaccine passport immediately and plans to get rid of mask requirements at the end of the month.

Alberta opposition leader Rachel Notley accused the province’s premier, Jason Kenney, of allowing an “illegal blockade to dictate public health measures.”

Despite Alberta’s plans to scrap its measures, the protest there continued.

“We’ve got guys here — they’ve lost everything due to these mandates, and they’re not giving up, and they’re willing to stand their ground and keep going until this is done,” said protester John Vanreeuwyk, a feedlot operator from Coaldale, Alberta.

“Until Trudeau moves,” he said, “we don’t move.”

As for the Ambassador Bridge blockade, Windsor Mayor Drew Dilkens said police had not removed people for fear of inflaming the situation. But he added: “We’re not going to let this happen for a prolonged period of time.”

The demonstration involved 50 to 74 vehicles and about 100 protesters, police said. Some of the protesters say they are willing to die for their cause, according to the mayor.

“I’ll be brutally honest: You are trying to have a rational conversation, and not everyone on the ground is a rational actor,” Dilkens said. “Police are doing what is right by taking a moderate approach, trying to sensibly work through this situation where everyone can walk away, nobody gets hurt, and the bridge can open.”

To avoid the blockade and get into Canada, truckers in the Detroit area had to drive 70 miles north to Port Huron, Michigan, and cross the Blue Water Bridge, where there was a 4½-hour delay leaving the U.S.

Interim Conservative leader Candice Bergen said in Parliament that countries around the world are removing restrictions and noted that Canadian provinces are, too. She accused Trudeau of wanting to live in a “permanent pandemic.”

“Many of the reasons that were previously to keep Canadians under restrictions are vanishing before our eyes,” Bergen said. “The prime minister needs to put his ego aside. He needs to do what’s right for the country. He needs to end the mandates. He needs to end the restrictions.”

Eddie Dunning is now an unemployed truck driver. He lost his job after refusing to be vaccinated and has since joined the protesters. He feels the protest is personal. He said he will continue protesting for “as long as it takes,” regardless of potential police intervention.

“I’ve got nothing to lose,” he said.

The protests have triggered similar demonstrations around the world. New Zealand, Australia, and France have protests underway. In New Zealand, officials have seen over 1,000 people parking trucks and cars outside of parliament and in Australia’s capital of Canberra, roughly a thousand people have shown up to demonstrate.