KYIV, Ukraine (AP) — Russian-backed separatists said Friday that a Ukrainian attack with U.S.-supplied missiles on a separatist eastern region hit a prison and killed 53 Ukrainian prisoners of war who were captured in Mariupol. There was no immediate confirmation of the attack from Ukraine.

The attack with HIMARS multiple rocket launchers on the prison in Olenivka, in the Russian-controlled Donetsk region, also injured 75 Ukrainian POWs, said Russian Defense Ministry spokesman Lt. Gen. Igor Konashenkov. Speaking before the latest update from the separatists, he put the death toll at 40.

Konashenkov described the strike as a “bloody provocation” aimed at discouraging Ukrainian soldiers from surrendering. He said that eight prison guards were also wounded by the shelling.

The attack could not be independently verified and officials in Ukraine have not yet commented on the reports. Ukrainian authorities in the Donetsk region said Russia has pressed on with the shelling of civilian targets in Ukrainian-held areas.

“The fighting in the region has been intensifying by the day, and civilians must evacuate while it’s still possible,” said Donetsk Gov. Pavlo Kyrylenko. “The Russian army doesn’t worry about civilian casualties. They are pummeling cities and villages in the region.”

The Ukrainian troops in Mariupol were taken prisoner after the fierce fighting for Ukraine’s Azov Sea port, where they had been holed up at the giant Azovstal steel mill. Their resistance has become a symbol of Ukrainian struggle against the Russian invasion that started on Feb. 24.

The Azov Regiment and other Ukrainian units defended the steel mill for nearly three months, clinging to its underground maze of tunnels. More than 2,400 surrendered in May under relentless Russian attacks from the ground, sea and air.

Scores of Ukrainian soldiers were taken to prisons in Russian-controlled areas such as the Donetsk region, a breakaway area in eastern Ukraine which is run by Russian-backed separatist authorities. Some have returned to Ukraine as part of prisoner exchanges with Russia, but families of others have no idea whether their loved ones are alive, or if they will ever come home.

In other developments:

— Ukrainian officials said Russian forces shelled the country’s second-largest city, Kharkiv.

City mayor Ihor Terekhov said a central part of the northeastern city was hit, including a two-story building and a higher education institution. Terekhov said the strike occurred just after 4 a.m. on Friday.

“The State Emergency Service is already working — they are sorting out the rubble, looking for people under them,” Terekhov said in a Telegram update.

— The Ukrainian presidential office said that at least 13 civilians were killed and another 36 were wounded in Russian shelling over the past 24 hours.

In the southern city of Mykolaiv, at least four people were killed and seven others were wounded when Russian shelling hit a bus stop.

“The Russians have changed their tactics because of the Ukrainian forces’ successes in the south. … They fire near a bus stop,” Mykolaiv Gov. Vitaliy Kim said.

The Russian barrage also hit a facility for distribution of humanitarian assistance, where three people were wounded, officials said.

Ukrainian officials also said at least four civilians were killed and another five were wounded in the eastern town of Bakhmut in the Donetsk region, which is the focus of the Russian offensive in the Donbas. More than 30 residential buildings and a kindergarten were damaged.